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All animal experiments were conducted in accordance with the guidelines of the Korean animal protection statute and approved by the institutional review committee. C57BL/6N mice (Oriental bio, Seoul, Korea) of 8 weeks of age were used in this study. The animals were housed in a general, temperature- and humidity-controlled, pathogen-free environment on a cycle 12 h light/dark, and were allowed free access to food and water. After elevation of axial flap based on lateral thoracic artery, those mice that had irregular vessel anatomy were excluded for study. In total, 120 mice were used. They were placed randomly into four groups (n = 30 per group): group A - Control (flap elevation with PBS injection), group B - platelet-rich plasma, or PRP (flap elevation with PRP injection), group C - ischemia (flap elevation with 4 hours of clamping), and group D - ischemia and platelet-rich plasma, or ischemia-PRP (flap elevation with PRP injection, followed by 4 hours of clamping)..
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